Read this first

Below you will find links to tutorials about the IMAGEGUI, the main program for MVA of images in the NB toolbox.

I have taken a lot of time creating step by step tutorials on how to use each function within the IMAGEGUI.

You must go through ALL tutorials in order to use the IMAGEGUI properly.

The tutorials are available as pdf downloads. In each case
the tutorials will take you step by step through how to use the functions of the gui.

For best performance the IMAGEGUI should be used on a high end PC. The more memory and faster CPU the better. The IMAGEGUI will cause Matlab to run out of memory if you import image data sets with a large number of peak images (>100 to 150 peaks). I am working on improving the performance, but this is not easy with Matlab.
If you run into 'Out of Memory' errors, try reducing the size of the data set by down binning the image, or reducing the number of peak images in the data set.
You must include the total counts image and sum of rest image when exporting .bif/.bif6/.bif3d files from the Iontof software.

IMAGEGUI tutorials

Updated Tutorials for V2.8!

These tutorials were created from screen shots that were 1034x934 pixels.
They should work fine on any modern computer monitor.

You can download all of the pdf tutorials in a zip file or view/save each of them individually. To view a pdf tutorial
click on the link. To save, right-click and choose 'save as'.

If you are going to use the standalone version please see this tutorial NBToolbox Standalone - Important Information

Download all imagegui pdf tutorials in a zip file

1. General Introduction

2. Importing Data

3. Normalizing Image Data

4. Cropping an Image

5. Filtering an Image

6. Image PCA

7. Plotting Image Scores

8. Exporting MVA data

9. Plotting Image Data

10. Making RGB Overlays

11. Making Scalebars on Images

12. Using the Image Properties Panel

13. Dice and Classify Image Function

14. Create Image Ratio Plot

15. One Way to Import From PHI RAW data

16. Creating and Labeling Loadings Plots

17. Cut Up Stage Raster

18. Align Images

19. Extract ROI

20. MCR

21. PC Data Browser

22. Plot Pseudo Spectrum

23. Image Processing

24. Import imzML Data

25. Correlated Image Finder

26. Auto ROI Extractor